Figure 1. Analysis of putative analog sensitive clones via PCR. (a.) A schematic of CDK12 exon 6 with the positions of the homologous repair template (blue box), the analog sensitive mutation (red star), and the PCR primers specific to the analog sensitive (AS) and wild type (WT) sequences; the PCR product is expected to be 560 bp. (b.) PCR of genomic DNA from 6 putative analog sensitive clones is visualized using a 1.5% agarose gel stained with EtBr; the top section of the gel displays the results using AS specific primers while the bottom section of the gel displays the results using the WT primers. Note clone #5 which appears to contain only analog-sensitive sequence.