Figure 2. Sequencing of the putative CDK12 analog sensitive cell line (clone #5, figure 1). Plasmids containing TA cloned PCR fragments from CDK12 genomic DNA surrounding exon 6 were submitted for Sanger sequencing. Alignment to the canonical sequence, the translated protein sequence of the ORF, and chromatographs are shown; exons are displayed in upper case and introns in lower case. The codon for F/G 813 is highlighted in blue. 8 TA clones were analyzed and only the two displayed sequences were observed (6 analog-sensitive and 2 wild-type with the premature stop codon). The * in the translation indicates a stop codon.