Figure 7. CDK12^as and parental (WT) cell line growth rates with and without the addition of 1-NM-PP1. (a.) Representative images of the parental and CDK12^as cell line after 24 hour treatment with 1-NM-PP1. Cells were passaged at 0.5×10⁶ in a 6 well plate in the presence of DMSO, 5, or 10 μM 1-NM-PP1 and images were taken 24 hours later. The white bar in the lower right of the images corresponds to 200 μm. (b.) CDK12^as and the parental cell line (WT) was plated in T25 flasks with and without the addition of 5 μM 1-NM-PP1. Cell counts were taken at the indicated times, addition of 1-NM-PP1 to the CDK12^as cell line slowed the cells growth rate significantly.