Figure 8. Western blots of parental (WT) and CDK12^as whole cell extracts using two Anti-Ser2P CTD antibodies. Cells are either treated with 10 μM 1-NM-PP1 (+) or DMSO (−) for the indicated period of time. The Anti-Ser2P antibodies are H5 (in red) and 3E10 (in green); Rpb2 is used as a loading control (lowermost panel – in red). Note the differential reactivity of the H5 and 3E10 antibodies toward Rpb1 after CDK12 inhibition.