Fig 2. Dose-response curves of gap junction inhibitors applied directly to the cuticle of adult female A. aegypti mosquitoes (carbenoxolone R² = 0.824, meclofenamic acid R² = 0.455 and mefloquine R² = 0.44).

Efficacy (dead and flightless mosquitoes) was assessed 24 h after application. Taking into consideration the average mass of an adult female mosquito (1.97 mg), the ED₅₀ for carbenoxolone is 8.57 μg/mg. The ED₅₀ values for meclofenamic acid and mefloquine are not determinable. Values are means ± SEM. n = 4–8 replicates of ten mosquitoes per dose tested.