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. 2015 Sep 1;10(9):e0137084. doi: 10.1371/journal.pone.0137084

Table 1. dsRNA template synthesis primers.

Each primer set consists of an innexin specific region for amplification of the target gene from plasmid, and the T7 promoter sequence (TAATACGACTCACTATAGGGAGA).

dsRNA Template Forward dsRNA Template Reverse
Inx1 TAATACGACTCACTATAGGGAGAGCGAAGCTGCAGAAGCTATT TAATACGACTCACTATAGGGAGAAAATGTTTTGTCGAGGTTCATGT
Inx2 TAATACGACTCACTATAGGGAGATTTGGCGTTTGAAAAGTGTG TAATACGACTCACTATAGGGAGAATACTCCCGGCTGAGCAATA
Inx3 TAATACGACTCACTATAGGGAGACGACGGTGACAGATTGACTAG TAATACGACTCACTATAGGGAGAGTTCGCTCCTGGTTGTACTC
Inx4 TAATACGACTCACTATAGGGAGACATTCCTGTTCTCGTTCCCC TAATACGACTCACTATAGGGAGAAAGGCACAGGGCATCAAAGT
Inx7 TAATACGACTCACTATAGGGAGACAGGGACAATCCAAAAGCATG TAATACGACTCACTATAGGGAGATCAGTTTCGTCAGCCTCATC
Inx8 TAATACGACTCACTATAGGGAGATTCTGACGATACTGACGACGTT TAATACGACTCACTATAGGGAGATCATGCATCCTGTATTTCACCT
eGFP TAATACGACTCACTATAGGGACGTAAACGGCCACAAGTT TAATACGACTCACTATAGGGTTGGGGTCTTTGCTCAGG