Table 1. Primer sequences used for qRT-PCR procedures.
Forward primer 5’ to3’ | Reverse primer 5’ to3’ | ProductSize | |
---|---|---|---|
NCAM-120 | GAACCTGATCAAGCAGGATGACGG | CTAACAGAGCAAAAGAAGAGTC | 321 bp |
NCAM-140 | GTCCTGCTCCTGGTGGTTGTG | CCTTCTCGGGCTCCGTCAGT | 264 bp |
NCAM-180 | CGAGGCTGCCTCCGTCAGCACC | CCGGATCCATCATGCTTTGCTCTC | 336 bp |
FGFR1 | GGCTACAAGGTCCGTTATGCC | GATGCTGCCGTACTCATTCTC | 105 bp |
WFDC2 (HE4) | AGAACTGCACGCAAGAGTG | TTGAGGTTGTCGGCGCATT | 52 bp |
α-SMA | AAGCACAGAGCAAAAGAGGAAT | ATGTCGTCCCAGTTGGTGAT | 76 bp |
SLUG | ACTCCGAAGCCAAATGACAA | CTCTCTCTGTGGGTGTGTGT | 119 bp |
SNAIL | GGCAATTTAACAATGTCTGAAAAGG | GAATAGTTCTGGGAGACACATCG | 105 bp |
MMP2 | TACAGGATCATTGGCTACACACC | GGTCACATCGCTCCAGACT | 90 bp |
MMP9 | TGTACCGCTATGGTTACACTCG | GGCAGGGACAGTTGCTTCT | 97 bp |
ALK3 | GGACATTGCTTTGCCATCATAG | GGGCTTTTGGAGAATCTTTGC | 112 bp |
BMP7 | CCTCCATTGCTCGCCTTG | TATGCTGCTCATGTTTCCTAATAC | 114 bp |