Fig 3. A20 protein expression in CpG/ CpG-siRNA_A20 treated BMDCs.
BMDCs on day 15 of culturing were treated with 1 nmol CpG/ CpG-scrambled siRNA/ CpG-siRNA A20 constructs (CpG-siA20_5, CpG-siA20_6). Protein lysates were collected 12, 16, 20, and 24 hours after treatment and analyzed by Western Blot using A20-specific antibody and actin as loading control.