Fig 2. Total amount of phospholipids in cell free homogenate from wild type and the Δgph1 deletion mutant.

Cells were grown on YPD to the early stationary growth phase at 30°C and disrupted with the aid of glass beads. Lipids were extracted with chloroform/methanol (2:1; v/v). Black bar, wild type BY4741; grey bar, Δgph1 mutant. (A) Total amounts of phospholipids were related to the amounts of protein. (B) Total amounts of phospholipids were related to cell wet weight. Inorganic phosphate was used as standard.