Fig 3. Synthesis of phosphatidylcholine is down regulated in the Δgph1 deletion mutant in vivo.

Black bar, wild type BY4741; grey bar, Δgph1 deletion mutant; (A) The so-called Kennedy pathway (CDP-choline pathways) was analyzed by incorporation of [methyl-¹⁴C]choline chloride into PC. (B) To analyze the methylation pathway of PC, the incorporation of L-[³H]serine into PS, PE and PC was measured. Wild type BY4741 was set at 100%.