Table 3. Phospholipid composition of cell-free homogenate, plasma membrane and mitochondria from cells grown on YPD or minimal medium (*).

CF, cellular fraction; LPL, lysophospholipids; PI, phosphatidylinositol; PS, phosphatidylserine; PE, phosphatidylethanolamine; PC, phosphatidylcholine; CL, cardiolipin; DMPE, dimethylphosphatidylethanolamine; PA, phosphatidic acid. WT [pYES2], wild type bearing plasmid pYES2; WT [pYES_gph1], overexpression of GPH1 on plasmid pYES in wild type background. Mean values of at least three measurements and standard deviations are shown.

		Phospholipids (mol%)
CF	Strain	LPL	PI	PS	PC	PE	CL	DMPE	PA	Others
Homogenate	WT [pYES2]*	1.9 ± 1.1	7.5 ± 1.0	8.0 ± 0.8	47.5 ± 3.9	24.5 ± 5.7	3.0 ± 0.9	1.1 ± 0.9	3.6 ± 1.4	3.1 ± 0.9
	WT [pYES_gph1]*	1.4 ± 0.9	7.5 ± 1.1	6.7 ± 0.9	53.2 ± 5.9	21.8 ± 1.4	3.2 ± 0.3	1.8 ± 0.3	2.8 ± 0.8	1.5 ± 0.5
	WT	1.5 ± 0.2	9.9 ± 3.5	8.8 ±.0.5	45.1 ± 1.8	26.6 ± 2.5	3.4 ± 0.3	4.4 ± 0.7	0.7 ± 0.4	0.0 ± 0.0
	Δglc3	0.1 ± 0.0	7.8 ± 3.2	5.4 ± 1.5	47.3 ± 5.1	30.4 ± 5.5	3.3 ± 1.2	4.3 ± 1.4	1.4 ± 0.8	0.0 ± 0.0
Mitochondria	WT [pYES2]*	3.5 ± 0.8	6.6 ± 1.3	3.9 ± 0.4	37.1 ± 8.6	39.6 ± 7.3	5.5 ± 1.9	0.3 ± 0.0	2.8 ± 2.2	0.8 ± 0.2
	WT [pYES_gph1]*	3.5 ± 0.7	6.1 ± 0.4	2.5 ± 0.7	41.5 ± 9.5	35.6 ± 6.2	7.3 ± 2.0	1.2 ± 1.0	1.5 ± 0.9	1.0 ± 0.5
	WT	1.9 ± 1.1	8.1 ± 1.7	4.1 ± 0.6	40.7 ± 2.4	30.4 ± 1.4	5.0 ± 3.6	6.6 ± 3.5	2.4 ± 0.4	0.7 ± 0.6
	Δglc3	0.6 ± 0.1	6.5 ± 2.7	2.9 ± 0.3	27.5 ± 5.0	41.0 ± 4.3	9.1 ± 4.3	4.3 ± 0.9	8.1 ± 1.2	0.0 ± 0.0
Plasma membrane	WT [pYES2]*	2.3 ± 0.7	4.7 ± 0.3	15.9 ± 0.5	35.4 ± 5.7	33.3 ± 5.1	0.0 ± 0.0	0.6 ± 0.4	4.1 ± 0.9	3.6 ± 0.2
	WT [pYES_gph1]*	8.2 ± 1.7	9.3 ± 5.2	24.9 ± 7.8	19.5 ± 6.5	31.0 ± 9.8	3.0 ± 0.5	0.0 ± 0.0	4.3 ± 1.0	0.0 ± 0.0
	WT	2.4 ± 1.0	12.4 ± 2.1	26.2 ± 2.8	18.2 ± 1.4	32.1 ± 2.1	0.7 ± 0.1	2.2 ± 0.4	5.8 ± 0.6	0.0 ± 0.0
	Δglc3	0.8 ± 0.2	7.7 ± 5.7	13.1 ± 2.3	42.3 ± 8.9	28.3 ± 6.5	0.5 ± 0.3	5.9 ± 2.9	1.4 ± 0.4	0.0 ± 0.0