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. 2015 Sep 1;10(9):e0137355. doi: 10.1371/journal.pone.0137355

Fig 6. Complementation of selected Pseudomonas syringae pv. phaseolicola transposon disruption mutants.

Fig 6

Genes identified through transposon (Tn) insertion were cloned into a broad host range vector and transformed into their respective mutant strain (TnC). An empty vector was also transformed into the strains to use as a control (TnE). A number of tests were carried out with these strains: A. in vitro growth in LB broth after 16hrs; B. in planta growth in bean cultivar Canadian Wonder after 2 days; C. colony size after 2 days incubation, shown at the same magnification; D. swarming ability in soft agar after 5 days incubation, shown at the same magnification. 14-, Pph 1448A; 13- Pph 1302A; CHP, conserved hypothetical protein. *above bars indicate significant differences compared to WT at p<0.05 assessed with Students t-test.