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. 2015 Sep 2;4:467. doi: 10.1186/s40064-015-1195-0

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© Zhuang et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — SDS-PAGE analysis of recombinant proteins expressed in P. pastoris GS115. Arrows indicate Eht1 (a) and Eeb1 (b). Lane 1 molecular mass standards; lane 2 culture medium of empty vector strain; lanes 3-7 culture medium of recombinant strain induced for 24, 48, 72, 96 and 120 h, respectively. Proteins in the polyacrylamide were stained with Coomassie Brilliant Blue R-250.