Table 3.

Protein concentration (mg/L) and esterase activity (U/mg) of recombinant enzymes

Sample	Protein concentration (mg/L)	Esterase activity (U/mg)a
Recombinant Eht1b	100.3 ± 10.3	74.5 ± 10.5
Recombinant Eeb1b	113.6 ± 9.7	81.6 ± 12.7
Controlc	7.7 ± 1.5	10.3 ± 0.7

^aEsterase activity was determined using p-nitrophenyl acetate as a substrate; protein yield was determined based on absorbance at 280 and 260 nm. Results are expressed as the mean ± standard deviation from triplicate experiments.

^bSample was collected from 120 h post-induction supernatant containing extracellular enzymes by recombinant P. pastoris.

^cSample was collected from 120 h post-induction supernatant by P. pastoris containing empty vector.