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Details of secondary antibodies used for IHC and WB
| Conjugate and host | Against | Dilution | Company | |
|---|---|---|---|---|
| IHC | ||||
| FITC (goat) | Anti-rabbit | 1:100 | Cayman Chemical, Ann Arbor, MI, USA | |
| Alexa 594 (goat) | Anti-mouse | 1:100 | Invitrogen, CA, USA | |
| WB | ||||
| HRP-conjugated | Anti-rabbit | 1:2000 | Cell Signaling Technology, Danvers, MA, USA | |
| HRP-conjugated | Anti-mouse | 1:2000 | Cell Signaling Technology, Danvers, MA, USA |
