Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Aug 11;5:688–704. doi: 10.1016/j.fob.2015.08.005

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 4 — Lower levels of proinflammatory cytokines in Git2^−/− mice after Con A injection. Git2^+/+ and Git2^−/− mice (n = 3) were injected in tail vein with 15 mg/kg Con A. (A) Serum levels of cytokine were determined at indicated time points after Con A injection by ELISA. (B) The mRNA levels of the cytokines in the hepatic homogenate were determined at indicated time points after Con A injection by real-time PCR. The expression levels for the target genes were normalized to GAPDH. The real-time PCR procedure was performed three times. (C) Liver MNCs were isolated 1 h after Con A injection and labeled with FITC- or APC-conjugated CD3 antibody following with stained with FITC-, PE-, APC-conjugated indicated antibodies and analyzed by FACS. The results were representative of three independent experiments and error bars represent standard deviations (^*P < 0.05, ^**P < 0.01, ^***P < 0.001).

Fig. 4 — Lower levels of proinflammatory cytokines in Git2^−/− mice after Con A injection. Git2^+/+ and Git2^−/− mice (n = 3) were injected in tail vein with 15 mg/kg Con A. (A) Serum levels of cytokine were determined at indicated time points after Con A injection by ELISA. (B) The mRNA levels of the cytokines in the hepatic homogenate were determined at indicated time points after Con A injection by real-time PCR. The expression levels for the target genes were normalized to GAPDH. The real-time PCR procedure was performed three times. (C) Liver MNCs were isolated 1 h after Con A injection and labeled with FITC- or APC-conjugated CD3 antibody following with stained with FITC-, PE-, APC-conjugated indicated antibodies and analyzed by FACS. The results were representative of three independent experiments and error bars represent standard deviations (^*P < 0.05, ^**P < 0.01, ^***P < 0.001).