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. 2015 Aug 11;5:688–704. doi: 10.1016/j.fob.2015.08.005

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© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 5 — Defective TCR-induced CD4⁺ T cell activation, proliferation, and cytokine production in the absence of GIT2. CD4⁺ T cells were purified from the spleens of the Git2^+/+ and Git2^−/− mice. (A) CD25 expression was measured by FACS at 24 h after 2.5 μg/ml Con A treatment as indicated. (B) The CD4⁺ T cells were stimulated with Con A, anti-CD3 and anti-CD28, PMA/ionomycin as indicated for panel added in vitro for 72 h respectively, and the proliferation was examined at 2 h after BrdU labeling. (C) 5 μM CFSE-labeled splenocyte were stimulated with anti-CD3/CD28 Abs for 72 h or with Con A for 5 d. Cells were harvested and gated on CD4⁺ T cells for FACS. Unstimulated, CFSE-labeled cells were used to verify the peak corresponding to the undivided population. (D) The cells were treated for 48 h as indicated for panel A, and supernatants were collected. Amounts of TNF-α, IFN-γ and IL-2 were determined by ELISA. (E) The cells were treated as indicated for 48 h, and supernatants were collected. Amounts of TNF-α, IFN-γ, IL-2, IL-4, IL-6, and IL-17 were determined by ELISA. The results were representative of three independent experiments and error bars represent standard deviations (^*P < 0.05, ^**P < 0.01, ^***P < 0.001).

Fig. 5 — Defective TCR-induced CD4⁺ T cell activation, proliferation, and cytokine production in the absence of GIT2. CD4⁺ T cells were purified from the spleens of the Git2^+/+ and Git2^−/− mice. (A) CD25 expression was measured by FACS at 24 h after 2.5 μg/ml Con A treatment as indicated. (B) The CD4⁺ T cells were stimulated with Con A, anti-CD3 and anti-CD28, PMA/ionomycin as indicated for panel added in vitro for 72 h respectively, and the proliferation was examined at 2 h after BrdU labeling. (C) 5 μM CFSE-labeled splenocyte were stimulated with anti-CD3/CD28 Abs for 72 h or with Con A for 5 d. Cells were harvested and gated on CD4⁺ T cells for FACS. Unstimulated, CFSE-labeled cells were used to verify the peak corresponding to the undivided population. (D) The cells were treated for 48 h as indicated for panel A, and supernatants were collected. Amounts of TNF-α, IFN-γ and IL-2 were determined by ELISA. (E) The cells were treated as indicated for 48 h, and supernatants were collected. Amounts of TNF-α, IFN-γ, IL-2, IL-4, IL-6, and IL-17 were determined by ELISA. The results were representative of three independent experiments and error bars represent standard deviations (^*P < 0.05, ^**P < 0.01, ^***P < 0.001).