Fig. 9.

The effect of isolated Tregs on proliferation of responder cells. CD4⁺CD25⁻ responders were labeled with CFSE and cultured alone or with CD4⁺CD25⁺ Tregs at a ratio of 2:1 in the presence of 5 μg/ml Con A. Cells were harvested and gated on CD4⁺CD25⁻ T cells for FACS. Unstimulated, CFSE-labeled cells were used to verify the peak corresponding to the undivided population.