Subject area	Environmental biology
More specific subject area	Amphipod comparative proteomics
Type of data	MS data, Tables
How data was acquired	Data-dependent acquisition of tandem mass spectra using a LTQ-Orbitrap-XL mass spectrometer (Thermo).,
Data format	.raw files,.mgf peak lists,.mzid identified files from MASCOT (Matrix science),.xls output data after validation with IRMA software.
Experimental factors	For each female, the ovary pair was dissected under stereomicroscope magnification, immediately frozen in liquid nitrogen and stored at −80 °C until needed. Proteins were extracted and analyzed by shotgun proteomics.
Experimental features	The 25 proteomes were briefly run on SDS-PAGE, followed by trypsin proteolysis. Tryptic peptides were analyzed by nanoLC-MS/MS and spectra were assigned with four protein sequence databases.
Data source location	CEA-Marcoule, DSV-Li2D, Laboratory “Innovative technologies for Detection and Diagnostics”, BP 17171, F-30200 Bagnols-sur-Cèze, France
Data accessibility	Deposited to the ProteomeXchange with identifiers PXD002253 forG. fossarum, PXD002309 forG. pulex, PXD002311 forG. roeseli, PXD002308 forH. Azteca, and PXD002310 forP. hawaiensis(http://proteomecentral.proteomexchange.org).