Figure 5.

Neuronal TFEB transduction reduces levels of APP, its CTFs, and Aβ species in wild-type mice. A, B, Immunoblot (A) with quantitation (B) of APP, and its α- and β-CTFs in wild-type mouse hippocampi transduced with AAV8-CMV-FLAG-TFEB (TFEB) or AAV8-CMV-GFP (GFP) at 8 months of age. N = 3 per group; *p < 0.05. C, Aβ40 and Aβ42 levels in dissected hippocampal tissues homogenized in RIPA buffer, from mice transduced as in A. HJ2 and HJ7.4 antibodies were used for capture Aβ40 and Aβ42, respectively, and HJ5.1 antibody was used for detection. N = 6 per group; *p < 0.05.