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. 2015 Sep 2;35(35):12241–12247. doi: 10.1523/JNEUROSCI.3937-14.2015

Figure 3.

Figure 3.

EGFR overexpression in TACE deficient OPs overcomes the defects in oligodendrogenesis and SCWM myelination during postnatal development. A, Immunofluorescence analysis of EGFR activation levels (pEGFR) in oligodendroglial cells (CC1+) at P15 in the SCWM of NG2-cre:TACEfl/fl:CNP-hEGFR mice. B–E, Immunofluorescence analysis and quantification of caspase3+ cells (B, C), proliferating OPs (NG2+BrdU+; E), pre-OLs (CC1+; D, E), and mature myelinating OLs (GSTπ+; D, E) in the SCWM at P15. F, Immunofluorescence analysis of MBP expression in the SCWM of NG2-cre:TACEfl/fl:CNP-hEGFR mice and their littermates at P15. G, Western blot analysis and quantification of myelin-related proteins levels in the SCWM of NG2-cre:TACEfl/fl:CNP-EGFR mice and their littermates at P15. Data are shown as mean ± SEM. n = 6–8 brains for each genotype. Cell numbers are expressed as the number of positive cells per mm3 (×1000). Scale bars: A, B, D, 20 μm; F, 100 μm. **p < 0.01 versus NG2-cre:TACEw/w; ##p < 0.01, ###p < 0.001 versus NG2-cre:TACEfl/fl mice.