Figure 4.

EGFR overexpression restores OL regeneration and CNS remyelination following demyelination in TACE^fl/fl mice. A, Adult NG2-cre:TACE^fl/fl:CNP-hEGFR:Rosa-YFP mice and their control littermates were fed cuprizone for 9 weeks and SCWM tissue was processed for histology and molecular analysis at 1 week after Cupz removal. Immunofluorescence analysis and quantification of caspase3⁺ cells (B), proliferating OPs (NG2⁺BrdU⁺ cells; D), pre-OLs (CC1⁺ cells; C), and mature myelinating OLs (GSTπ⁺, MBP; C, D, F) in the SCWM of NG2-cre:TACE^fl/fl:CNP-hEGFR:Rosa-YFP mice at 1 week after recovery. WB (D) and IF (E, F) analysis and quantification of myelin related protein levels at 1 week after recovery. EGFR overexpression rescues the delay in SCWM remyelination observed in TACE^fl/fl mice. Data are shown as mean ± SEM. n = 6–8 brains for each genotype. Cell numbers are expressed as the number of positive cells per cubic millimeter (×1000). Scale bars: E, 100 μm; F, 10 μm. **p < 0.01 versus NG2-cre:TACE^w/w mice; ##p < 0.01 versus NG2-cre:TACE^fl/fl mice.