TABLE 2 .
Mobilization frequency in the presence of 2-HDA and 2-ODA
| Plasmidb | Incc | MOBd | MPFe | MF (%)a |
||||
|---|---|---|---|---|---|---|---|---|
| 2-HDA (mM) |
2-ODA (mM) |
|||||||
| 0.2 | 0.4 | 1 | 0.2 | 0.4 | ||||
| CloDF13 (R388) |
ColE | C11 | −(T) | 12** | 9*** | 2*** | 29 | 26* |
| ColE1 (pOX38) |
ColE | P5 | −(F) | 8** | 4*** | 3*** | 31* | 11** |
| ColE1 (pRL443) |
ColE | P5 | −(T) | 100 | 77 | 70 | 100 | 87 |
| RSF1010 (pRL443) |
Q1 | Q11 | −(T) | 84 | 58 | 59 | 70 | 66 |
a
Mobilization frequencies (MF) in the presence of 2-HDA and 2-ODA using three different mobilizable plasmids, expressed as a percentage relative to a control without added COINs (100%). Values represent means of the results of at least four independent experiments measured by plate conjugation assay. *, P < 0.05 (mean significantly different from control); **, P < 0.01; ***, P < 0.001.
b
The helper plasmids used are indicated in parentheses.
c
Inc, incompatibility group (38).
d
MOB, MOB group (39).
e
MPF, mating pair formation type (40). The minus signs represent the absence of MPF in mobilizable plasmids, which use helper MPF (in parentheses).