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. 2015 Sep 1;6(5):e01188-15. doi: 10.1128/mBio.01188-15

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Copyright © 2015 McDonald et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 4 — Lipid vesicles are able to derepress transcription and disrupt a PspA-PspF inhibitory complex. (a) Denaturing gels, showing that production of the spRNA product (UpGGG) in the presence of the transcriptional activator PspF (lane 2) is inhibited upon addition of PspA (lane 3). Repression was relieved with the addition of TLE vesicles (lane 4). (b) spRNA production with incorporation of an increasing SCE stress vesicle set. Increasing membrane stored energy composition levels are shown, from left to right: DMPC/DOPC 4:6, DMPC/DOPC 2:8, DOPC, DOPE/DOPC 2:8, DOPE/DOPC 4:6. (c) Recovery of spRNA production plotted for each vesicle composition at 1 mM lipid concentration.