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Sephadex G-75 chromatography of pooled CSF extracts (top). Fractions were assayed for POMC (●) by ELISA and ACTH (gray triangles) by RIA. Arrows indicate the void volume (Vo) and the elution positions of POMC and ACTH standards. HPLC of a CSF pool (bottom) with fractions assayed for AgRP by RIA. Arrows indicate the elution positions of full-length AgRP and AgRP83–132 standards.
