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Sucrose density gradient and Western blot (WB) analysis of MARCKS and MARCKS-like protein 1 (MLP-1) in response to CaMKII inhibition in Xenopus 2F3 cells. Western blot analysis probing for MARCKS and MLP-1 shows the total amount of MARCKS and MLP-1 protein did not change, but the protein density shifted from heavy to light fractions. A greater amount of MARCKS and MLP-1 protein appear in lanes 1 and 4 with KN93 treatment (0.5 μM) compared with the vehicle (MOCK) treatment.
