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Deletion of the α-actinin-binding site in ASIC-1 prevents coimmunprecipitation of ASIC-1 and integrin-β1. Immunoprecipitation of lysates from D54MG (A) and U373 (B) cells with antibodies directed against ASIC-1 and immunoblotting for integrin-β1 showed significant inhibition of association of ASIC-1 with integrin-β1 in the mutant cells (n ≥ 4).
