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Inhibition of ERK1/2 phosphorylation. Immunoblot analysis of lysates from D54MG (A) and U373 (B) cells stably transfected with shRNA for scrambled or KD shRNA for α-actinin-1 or -4 or transfected with the ASIC-1 COOH-terminal mutant. Blots were probed for phosphorylated ERK1/2 and then stripped and reprobed for total ERK1/2 (n ≥ 3). Phosphorylation of ERK was significantly inhibited in each case compared with cells expressing WT ASIC-1 (n ≥ 6). **P < 0.01; ***P < 0.001.
