Fig. 2.

Knockdown of integrin-β₁ in glioma cells. A: lysates from untransfected wild-type (WT) D54MG cells and D54MG cells stably transfected with shRNA for integrin-β₁ [knockdown (KD)], scrambled shRNA (Scr), or empty vector (EV). Expression of integrin-β₁ was significantly decreased in KD cells compared with WT cells (n ≥ 4). B: expression of integrin-β₁ in 3 additional glioma cell lines (U87, U251, and U373) was also significantly reduced by shRNA. ***P < 0.001 by ANOVA and Dunnett's post hoc test (A) and Student's t-test (B).