Fig. 5.

Cell adhesion through fibronectin increased membrane expression of ASIC-1. A: total and surface proteins were immunoblotted for GFP and integrin-β₁ in D54MG cells. Membrane localization of ASIC-1 was significantly increased and total and membrane expression of integrin-β₁ was higher following 24 h of incubation on fibronectin (FN)-coated plates (n ≥ 3). B: normalized densitometry of membrane ASIC-1 and integrin-β₁ in cells incubated on fibronectin-coated dishes (FN+) or on uncoated plates (FN−) for 24 h. β-Actin was used as a loading control. *P < 0.05; ***P < 0.001.