Skip to main content
. 2015 Jun 24;309(5):C296–C307. doi: 10.1152/ajpcell.00022.2015

Fig. 7.

Fig. 7.

Apex-1 promotes AP-1 activation and c-Jun DNA binding. A and B: AP-1 transcription activity was analyzed in EOMA cells. A: cells were transfected with control and Apex-1 siRNA; after 72-h transfection, cells were washed and cotransfected with a pAP-1 luciferase and pRL-TK Renilla luciferase plasmids for 48 h. Firefly luciferase activity levels were normalized with Renilla luciferase. B: AP-1 activity was measured in E3330 (50 μM, 5 h)-treated EOMA cells. C: association of c-Jun with Apex-1 was identified by coimmunoprecipitation (IP). The cell lysate were subjected to immunoprecipitation with Apex-1 antibody. The immunoprecipitates were subjected to SDS PAGE and immunoblotting (IB) for c-Jun and Apex-1. D and E: c-Jun DNA binding ability was measured in Apex-1 knockdown (D) and also in E3330 (50 μM, E)-treated EOMA cells using an ELISA-based (Trans-AM) method. F: treatment of CRT0044876 did not show any significant effects on c-Jun DNA binding activity. Results are expressed as means ± SD; *P < 0.05.