APOL1 mutagenic oligonucleotides for oocyte experiments. |
APOL1 mutant |
Mutagenic oligonucleotides |
Starting from APOL1 “WT”: |
|
APOL1.ΔBH3 |
F 5′-ctccgtgcccttgcagctggggttcagaaggtc-3′ |
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R 5′-gaccttctgaaccccagctgcaagggcacggag-3′ |
APOL1.D163A |
F 5′-ctccgtgcccttgcagctggggttcagaaggtc-3′ |
|
R 5′-gaccttctgaaccccagctgcaagggcacggag-3′ |
Starting from APOL1.D163A: |
|
APOL1.L158A/D163A/V165A |
F 5′-gaagggcccgtgcacttgcagctggggctcagaaggtccacaaag-3′ |
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R 5′-agccccagctgcaagtgcacgggcccttcttatgttatcctcaag-3′ |
Starting from APOL1.L158A/D163A/V165A: |
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APOL1.9ALA |
F 5′-cataagaagggccgctgcagctgcagctgcggctgcaaaggtccacaaag-3′ |
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R 5′-ctttgtggaccttcgcagccgcacgtgcagctgcagcggcccttcttatg-3′ |
BCL2 family member oligonucleotides for RP-PCR. |
Human BCL2 Family Member |
PCR oligonucleotides |
BCL2 |
NM_000633 |
F 5′-ccggaattcctctgggaaggatggcgcac-3′ |
|
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R 5′-tgctctagattggggcaggcatgttgacttc-3′ |
BCLXL |
NM_138578 |
F 5′-ccggaattcgagactcagtgagtgagcagg-3′ |
|
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R 5′-tgctctagagggagggtagagtggatggtcag-3′ |
BCLW |
NM_004050 |
F 5′-cacggaattcattaagagctgccatcccggctg-3′ |
|
|
R 5′-tgctctagacctggccctggactttcacttgc-3′ |
BCL2A1 |
NM_004049 |
F 5′-ccggaattctccaccaggcagaagatgacag-3′ |
|
|
R 5′-tgctctagagtcctttctggtcaacagtattgc-3′ |
MCL-1 |
NM_021960 |
F 5′-ccggaattcggcgactggcaatgtttggcctc-3′ |
|
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R 5′-tgctctagactaggaagttacagcttggagtcc-3′ |
APOL1 mutagenic oligonucleotides for PRG977 mouse expression vector. |
APOL1 mutant |
Mutagenic oligonucleotides |
Starting from APOL1 “WT”: |
|
APOL1.ΔBH3 |
F 5′-gaggataacataagaaggaaggtccacaaaggcacc-3′ |
|
R 5′-ggtgcctttgtggaccttccttcttatgttatcctc-3′ |
Starting from APOL1 “WT”: |
|
APOL1.L158A |
F 5′-gaggataacataagaagggcccgtgcccttgcagatg-3′ |
|
R 5′-catctgcaagggcacgggcccttcttatgttatcctc-3′ |
Starting from APOL1 “WT”: |
|
APOL1.R159A |
F 5′-ataacataagaaggctcgctgcccttgcagatggg-3′ |
|
R 5′-ccccatctgcaagggcagcgagccttcttatgttat-3′ |
Starting from APOL1 “WT”: |
|
APOL1.D163A |
F 5′-ccgtgcccttgcagctggggttcagaag-3′ |
|
F 5′-ccgtgcccttgcagctggggttcagaag-3′ |
Starting from APOL1.D163A: |
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APOL1.L158A/R159A/D163A: |
F 5′-tgaggataacataagaagggccgctgcccttgcagctgggg-3′ |
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F 5′-tgaggataacataagaagggccgctgcccttgcagctgggg-3′ |