Table 1.
Mutagenic oligonucleotides
| APOL1 mutagenic oligonucleotides for oocyte experiments. | ||
| APOL1 mutant | Mutagenic oligonucleotides | |
| Starting from APOL1 “WT”: | ||
| APOL1.ΔBH3 | F 5′-ctccgtgcccttgcagctggggttcagaaggtc-3′ | |
| R 5′-gaccttctgaaccccagctgcaagggcacggag-3′ | ||
| APOL1.D163A | F 5′-ctccgtgcccttgcagctggggttcagaaggtc-3′ | |
| R 5′-gaccttctgaaccccagctgcaagggcacggag-3′ | ||
| Starting from APOL1.D163A: | ||
| APOL1.L158A/D163A/V165A | F 5′-gaagggcccgtgcacttgcagctggggctcagaaggtccacaaag-3′ | |
| R 5′-agccccagctgcaagtgcacgggcccttcttatgttatcctcaag-3′ | ||
| Starting from APOL1.L158A/D163A/V165A: | ||
| APOL1.9ALA | F 5′-cataagaagggccgctgcagctgcagctgcggctgcaaaggtccacaaag-3′ | |
| R 5′-ctttgtggaccttcgcagccgcacgtgcagctgcagcggcccttcttatg-3′ | ||
| BCL2 family member oligonucleotides for RP-PCR. | ||
| Human BCL2 Family Member | PCR oligonucleotides | |
| BCL2 | NM_000633 | F 5′-ccggaattcctctgggaaggatggcgcac-3′ |
| R 5′-tgctctagattggggcaggcatgttgacttc-3′ | ||
| BCLXL | NM_138578 | F 5′-ccggaattcgagactcagtgagtgagcagg-3′ |
| R 5′-tgctctagagggagggtagagtggatggtcag-3′ | ||
| BCLW | NM_004050 | F 5′-cacggaattcattaagagctgccatcccggctg-3′ |
| R 5′-tgctctagacctggccctggactttcacttgc-3′ | ||
| BCL2A1 | NM_004049 | F 5′-ccggaattctccaccaggcagaagatgacag-3′ |
| R 5′-tgctctagagtcctttctggtcaacagtattgc-3′ | ||
| MCL-1 | NM_021960 | F 5′-ccggaattcggcgactggcaatgtttggcctc-3′ |
| R 5′-tgctctagactaggaagttacagcttggagtcc-3′ | ||
| APOL1 mutagenic oligonucleotides for PRG977 mouse expression vector. | ||
| APOL1 mutant | Mutagenic oligonucleotides | |
| Starting from APOL1 “WT”: | ||
| APOL1.ΔBH3 | F 5′-gaggataacataagaaggaaggtccacaaaggcacc-3′ | |
| R 5′-ggtgcctttgtggaccttccttcttatgttatcctc-3′ | ||
| Starting from APOL1 “WT”: | ||
| APOL1.L158A | F 5′-gaggataacataagaagggcccgtgcccttgcagatg-3′ | |
| R 5′-catctgcaagggcacgggcccttcttatgttatcctc-3′ | ||
| Starting from APOL1 “WT”: | ||
| APOL1.R159A | F 5′-ataacataagaaggctcgctgcccttgcagatggg-3′ | |
| R 5′-ccccatctgcaagggcagcgagccttcttatgttat-3′ | ||
| Starting from APOL1 “WT”: | ||
| APOL1.D163A | F 5′-ccgtgcccttgcagctggggttcagaag-3′ | |
| F 5′-ccgtgcccttgcagctggggttcagaag-3′ | ||
| Starting from APOL1.D163A: | ||
| APOL1.L158A/R159A/D163A: | F 5′-tgaggataacataagaagggccgctgcccttgcagctgggg-3′ | |
| F 5′-tgaggataacataagaagggccgctgcccttgcagctgggg-3′ | ||