Fig. 2.
TdT-mediated dUTP nick end labeling (TUNEL) immunofluorescence. Lung tissues were formalin fixed and paraffin embedded. Tissue sections were treated as described in methods, and photomicrographs were taken using fluorescence imaging (A). The green fluorescent cells (indicative of cell death) were counted and the data graphed (B). A total of n = 4 mice were assessed in each group with no more than 1 mouse per litter from each treatment group. The data were analyzed by 2-way ANOVA with Tukey's post hoc test. An effect of treatment, an effect of diet, and an interaction were indicated. Data are means ± SE and were analyzed for the effects of LPS/O2 exposure and diet. *P < 0.05, different from CD-saline/RA. $P < 0.05, different from DHA-LPS/O2; #P < 0.05, different from CD-LPS/O2.