Fig. 5.

Analysis of key intracellular and membrane cholesterol transporters and examination of proteins involved in lipoprotein packaging by QrtPCR and Western blotting. QrtPCR was determined as in experimental materials to determine relative mRNA abundance of cholesterol efflux transporters Abcg5 (A) and Abcg8 (B). 18S rRNA was used to normalize mRNA expression levels. Western blots of liver homogenates isolated from WT and DKO mice were performed analyzed as in experimental materials to determine relative protein levels of SCP-2 (C), SCP-X (D), liver fatty acid binding protein (L-FABP; E), ATP-binding cassette transporter A1 (ABCA1; F), ApoA1 (G), ApoB (H), and microsomal triglyceride transfer protein (MTP; I). COX4 was used as a loading control to normalize protein expression. E–H, insets: representative Western blots were spliced from multiple blots showing relative protein expression in each mouse group, with the same matching representative COX4 captures from the corresponding blot shown in multiple figures. Solid bars: WT mice; open bars: DKO mice. Values are means ± SE (n = 4–7). *P < 0.05 for DKO vs. WT. #P < 0.05 CH vs. CO.