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. 2015 Jun 25;309(5):G387–G399. doi: 10.1152/ajpgi.00460.2014

Fig. 6.

Fig. 6.

Effect of SCP-2/SCP-X gene ablation and cholesterol-rich diet on key regulators of fatty acid biosynthesis. Western blotting of liver homogenates isolated from WT and DKO mice were analyzed to determine relative protein levels of the 68-kDa sterol response element binding protein-1 (SREBP1; A) and 125-kDa pre-SREBP1 (B). COX4 was used as a loading control to normalize protein expression. QrtPCR was used to determine relative mRNA abundance of Srebp1 (C), Acc1 (D), Acc2 (E), and Fas (F). 18S rRNA was used to normalize mRNA expression levels. mRNA and protein expression levels were as described in experimental materials. A and B, insets: representative Western blots were spliced from multiple blots showing relative protein expression in each mouse group, with the same matching representative COX4 captures from the corresponding blot shown in multiple figures. Solid bars: WT mice; open bars: DKO mice. Values are means ± SE (n = 4–7). *P < 0.05 for DKO vs. WT. #P < 0.05 CH vs. CO.