Fig. 4.

Western blot analysis was unable to detect induction of mitophagy after alcohol treatment. A and B: WT and Parkin KO mice were treated with alcohol by using the acute-binge (A) and Gao-binge (B) models, and liver cytosolic (Cyto) and heavy membrane (HM) fractions were isolated and analyzed by Western blot. β-Actin or Gapdh and VDAC or Tom20 were used as loading controls. C and D: WT and Parkin KO mice were treated with alcohol by using the acute-binge (C) or Gao-binge (D) model, and liver lysates were used for Western blot analysis. β-Actin was used as a loading control. E and F: densitometry quantification for blots in C and D, respectively. Data shown are means ± SE (n = 3 mice per group; no significant differences among groups by 1-way ANOVA). G: WT and Parkin KO mice were treated with alcohol by using the acute-binge or Gao-binge models, and HM fractions were used for Western blot analysis. Cox II was used as a loading control.