Fig. 7.

Mitochondrial respiration rates and cytochrome c oxidase (COX) activity were decreased in Parkin KO mice after alcohol treatment. A–C: WT and Parkin KO mice were treated with alcohol by using the acute-binge model, and state 3 (A), state 4 (B), and maximal respiratory capacity (C) respiration rates were measured by Oroboros using isolated liver mitochondria. Data shown are means ± SE (n ≥ 3 mice per group; *P < 0.05 compared with WT alcohol by t-test). D and E: COX activity for WT and Parkin KO mouse livers treated with acute-binge (D) or Gao-binge (E). Data shown are means ± SE (n ≥ 3 mice per group; *P < 0.05 compared with WT control by 1-way ANOVA).