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. 2015 Sep 2;5:13677. doi: 10.1038/srep13677

Figure 2. miR-33a knockdown induces EMT and metastasis and miR-33a overexpression blocks EMT and metastasis.

Figure 2

(A) SPC-A-1 cells was transfected with miR-33a inhibitors (anti-miR-33a) or inhibitor NC (Ctrl RNA) and (B) NCI-H1299 cells was transfected with miR-33a mimics (miR-33a) or mimics NC (Ctrl RNA), the levels of mature miR-33a were confirmed by quantitative RT-PCR. Western Blotting was performed after the transfection of RNAs. The changes of epithelial cell biomarker E-cadherin and mesenchymal cell biomarker Vimentin were shown in SPC-A-1 (C) and NCI-H1299 (D) cells. The migration and invasion capability were respectively measured after the transfection of RNA oligos in SPC-A-1 (E,G) and NCI-H1299 (F,H) cells. *P < 0.05, **P < 0.01, ***P < 0.001.