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. 2015 Sep 2;5:13677. doi: 10.1038/srep13677

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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NCI-H1299 cells subjected to Twist1 siRNA knockdown and Western blotting was performed after 48h. The changes of epithelial cell biomarker E-cadherin and mesenchymal cell biomarker Vimentin and the semi-quantitative analyses were shown (A). The migration (B) and invasion (C) capability were measured after transfections of siRNA. *P < 0.05, **P < 0.01, ***P < 0.001.