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(A) MT-1, -2 detection by confocal microscopy in control and HepG2 cells treated with 2.5 μM and 5 μM crambescin C1 (CC1) for 12 h. Representative photos of control and treated cells are shown. Hoechst 33258 was used for nuclei counterstaining (blue) and quantification of nuclear metallothioneins (MTs). Arrows: MTs translocation to the nucleus in treated cells; (B) Quantification of the variations caused by CC1 in the levels of nuclear MTs.
