Figure 3.

6-acetylmonodethiogliotoxin inhibits TNFα-induced NF-κB-dependent ICAM-1 gene expression. 6-acetylmonodethiogliotoxin (Compound 1) inhibits NF-κB-dependent ICAM-1 genes expression. K562 cells were transiently transfected with ICAM-1 along with ph-RG-tk Renilla plasmid for 24 h. After transfection, K562 cells were treated or not with 6-acetylmonodethiogliotoxin at IC₅₀ concentrations for two hours followed by a TNFα-treatment (20 ng/mL) during 6 h. The cells were assayed for Luciferase activity. Each value is a mean ± SD of three determinations. Asterisks indicate a significant difference compared to control positive as analyzed by t-test (* p < 0.05; ** p < 0.01). Negative control (Co−) corresponds to transfected and DMSO only treated cells, without TNFα activation, positive control (Co+) corresponds to transfected and DMSO treated cells activated by TNFα.