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Effect of 6-acetylmonodethiogliotoxin on the degradation of IκBα and translocation of p65 and p50 to the nucleus. Jurkat cells were pre-treated with 6-acetylmonodethiogliotoxin (Compound 1) at 80 μM for 2 h followed by activation with TNFα (20 ng/mL) for indicated time periods. Cytoplasmic and nuclear extracts were tested for IκBα, pIκBα, p50 and p65. Protein loading and purity of extracts were verified by lamin B and α-tubulin Western blots.
