Table 3.
Advantages and disadvantages of the investigated cell disruption methods.
| Cell disruption methods | Advantages | Disadvantages |
|---|---|---|
| Manual grinding | - Quickest and efficient - 2 min process |
- Localised heating caused denaturation of molecules |
| Bead vortexing | - Can be established easily and relatively effective | - High heat generation, - Incomplete cell lysis |
| Osmotic shock | - Lower energy consumption - Easier scale-up |
- Generation of waste salt water - Time consuming |
| Water bath | - Maximum disruption - Easy in handling at lab scale |
- Increases the viscosity - Energy intensive |
| Sonication | - Faster extraction - Suitable for all cell type |
- Damage chemical structure of molecules |
| Shake mill | - Rapid method | - High energy intensive - High heat generation |