Figure 3.

The inhibitory concentration of Dicathais orbita crude extract and purified compounds required to reduce cell viability by 50% (IC₅₀ mg/mL on a log scale) in a range of cell lines using tetrazolium salt (MTT or MTS) cell proliferation assays; female reproductive cancer cells JAr, OVCAR-3 and KGN, as well as primary derived (healthy) granulosa cells after 4 h exposure [69]; colon cancer HT29 and Caco-2 cells after 12 h exposure [68]; lymphoma U937 and freshly isolated human mononuclear cells after 4 h exposure [51,105]. For freshly isolated human mononuclear cells (MNC), the IC₅₀ exceeded the maximum test concentration (1000 µg/mL) for the crude extract and semi-purified fractions after 4 h exposure [104], therefore results are presented for MNC viability after 60 min exposure using Trypan blue staining [126].