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. 2015 Aug 20;13(8):5334–5357. doi: 10.3390/md13085334

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Structure of the transformation vector. Abbreviations used in this map: PtfcpA pro.: the promoter region of FCP gene A derived from P. tricornutum UTEX 646; Ptpsy: P. tricornutum PSY gene isolated from P. tricornutum NRIA-0065; egfp: eGFP gene; CffcpA ter.: the terminator region of FCP gene derived from Cyl. fusiformis; CffcpA pro.: the promoter region of FCP gene derived from Cyl. fusiformis; Sh ble: antibiotic Zeocin™ resistance gene isolated from a mutant strain of Streptomyces verticillus; (P-ble) denotes the destination vector produced from pCfcp-ble [15].