Table 1.
Overview of sample collection and analysis.
| Sample codes | Collection location (date) | Air temp. montha (°C) | Water temp. (°C) | 16S rRNA clone library | Species-specific PCR | cDNA | Isolates |
|---|---|---|---|---|---|---|---|
| LAB I | MIT Laboratory (2008 and 2009) | 21 to 23 | 21 to 23 | √ | √ | √ | √ |
| MA-I | Sippewissett Marsh, MA (July, 2008) | 16 to 31 | 22b | √ | |||
| CT | Clinton, CT (July, 2008) | 15 to 34 | ND | √ | |||
| MB | Mahone Bay, NS (Sept, 2008) | 4 to 25 | ND | √ | |||
| MA-II | Sippewissett Marsh, MA (Nov. 2008) | −7 to 20 | 6 | √ | |||
| SED-MA-II | Sippewissett Marsh, MA (Nov. 2008) | −7 to 20 | 6 | √ | |||
| MA-III | Sippewissett Marsh, MA (June, 2009) | 5 to 28 | 20 | √ | |||
| SED-MA-III | Sippewissett Marsh, MA (June, 2009) | 5 to 28 | 20 | √ | |||
| WATER-MA-III | Sippewissett Marsh, MA (June, 2009) | 5 to 28 | 20 | √ | |||
| MA-IV | Sippewissett Marsh, MA (July, 2009) | 8 to 29 | 20 | √ | √ | ||
| (cDNA) | |||||||
| LAB II | MIT Laboratory (2010) | 21 to 23 | 21 to 23 | √ | |||
| MA-V | Sippewissett Marsh, MA (March-April, 2010) | −8 to 20 | ND | √ |
a
Monthly air temperature ranges were obtained from weather series of Cape Cod Air Station, MA (12 miles away from the Great Sippewissett Marsh), Clinton, CT, and Western Head, NS (42 miles from Mahone Bay, NS).
b
Water temperature was measured by an Onset® temperature logger. ND: Not Determined.