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PC12 cells incubated for 72 h with reduced media (A), reduced media modified with NGF (B), reduced media modified with NGF-PEI-NP (C), or reduced media modified with NGF:PEI-NP (D) (20X). Confocal microscopy of PC12 cells after 72 h of incubation with NGF:PEI-NPs is shown in (E). Actin staining reveals the presence of NPs (green) in cell body, the growth cone at the tip of the axons of the differentiated cells (40X).
