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Double fluorescence detection of GFAP (red; A,E) and aromatase B (green; B,F) along the ventricular surface in female goldfish telencephalon. The confocal images show expression of both aromatase B and GFAP along the ventricular surface (V; ventricle). The merged images show colocalization of aromatase B and GFAP in RGCs (D,H). The nuclear stain DAPI (blue) is also shown (C,G). Scale bar = 20 μm.
