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. 2015 Sep 2;9:310. doi: 10.3389/fnins.2015.00310

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Copyright © 2015 Xing, McDonald, Da Fonte, Gutierrez-Villagomez and Trudeau.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Double fluorescence detection of GFAP (red) and TH (green) in ventral telencephalic area of the female goldfish brain. The confocal images show RGC cell bodies marked by GFAP located along the ventricular surface (A,E). Some catecholaminergic neuron cell bodies marked by TH are located along the ventricular surface (B,F). Magnification of the boxed area in (D) shows that there are very close anatomical relationships between RGC fibers and catecholaminergic cell bodies (H). The confocal image shows RGC cell bodies marked by GFAP located along the ventricular surface with fibers extending from ventricular surface to the area ventralis telencephali pars ventralis (I,M). Catecholaminergic neuron cell bodies marked by TH are located at preoptic periventricular nucleus (NPP) with fibers extending to the area ventralis telencephali pars ventralis (J,N). Magnification of the boxed area in (L) shows that there are very close anatomical relationships between catecholaminergic neuronal processes and RGC fibers (V; ventricle, H,P). The nuclear stain DAPI (blue) is also shown (C,G,K,O). Scale bar = 20 μm.